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KMID : 0603820070130010071
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Journal of Experimental & Biomedical Science
2007 Volume.13 No. 1 p.71 ~ p.73
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Multiplex PCR Assay from Colon Biopsy Specimens in Acute Infectious Colitis Patients
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Lee Gyu-Sang
Lim Kwan-Hun
Kim Jong-Bae
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Abstract
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For the rapid detection of objective pathogenic bacteria from colon biopsy specimens, multiplex PCR (polymerase chain reaction) method was developed. The major objective bacteria in this study were Shiga-like toxin producing E. coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella spp. Salmonella spp. and Yersinia spp.. To detect simultaneously 7 kinds of pathogenic bacteria in single reaction tube, multiplex PCR system was executed using 6 sets of primers used in single PCR system for the respective bacteria. The results in this research might be applied for the detection of pathogenic bacteria form colon biopsy samples.
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KEYWORD
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Colon biopsy specimen, Multiplex PCR
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